Single-site substitutions improve cold activity and increase thermostability of the dehairing alkaline protease (DHAP)
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چکیده
منابع مشابه
Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives
A fungal strain (Conidiobolus brefeldianus MTCC 5184) isolated from plant detritus secreted a high activity alkaline protease. Thermostability studies of the fungal alkaline protease (FAP) revealed that the protease is stable up to 50°C with 40% residual activity after one hour. Effect of various additives such as sugars, sugar alcohols, polyols, and salts, on the thermostability of FAP was eva...
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The purpose of the present investigations was to improve the yield of alkaline protease for leather dehairing by subjecting the indigenous proteolytic strain Bacillus licheniformis N-2 to various mutagenic treatments viz. UV irradiations, NTG (N-methyl-N-nitro-N-nitrosoguinidine) and MMS (methyl methane sulfonate). After screening on skim milk agar plates, a total of nine positive mutants were ...
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subtilisins and proteinase K.]) Many protein cngincering studjes on subtilisins6) show that amino acid substitution can change the substrate specificity, pH depencience. thermostability, and eatalytie activity. Subtilisin BPN' mutant N218S (Asn218 is rcplaced by serine) has more thermostability than the wild-type cnzyme;7) Asn218 is threc rcsidues fi'om one of the catalytic residues. Ser221. Th...
متن کاملAlkaline Ribonuclease Activity Increase in Rat
Acid azo dyes, most of them naphtholdisulfonic acid derivatives, were given intraperitoneally to rats and their effect on "alkaline" ribonuclease activity was studied in total homogenates of kidney cortex and liver. Acid treatment was used to release bound enzyme activity. Several of the dyes, including trypan blue, increased RNase activity in both organs 3 days after administration of single d...
متن کاملAlkaline Ribonuclease Activity Increase in Rat
Acid azo dyes, most of them naphtholdisulfonic acid derivatives, were given intraperitoneally to rats and their effect on "alkaline" ribonuclease activity was studied in total homogenates of kidney cortex and liver. Acid treatment was used to release bound enzyme activity. Several of the dyes, including trypan blue, increased RNase activity in both organs 3 days after administration of single d...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2016
ISSN: 0916-8451,1347-6947
DOI: 10.1080/09168451.2016.1230005